automated patch-clamp platform qpatch 16 Search Results


hek293 cells heterologously expressing human na v 1.7  (SB Drug Discovery)
90
SB Drug Discovery hek293 cells heterologously expressing human na v 1.7
(A) Sample traces of the fluorescence levels of Na V <t>1.7-HEK</t> cells with ANG-2 and PSS buffer addition, with and without washing, obtained using the FLIPR TETRA . (B) Sample traces of the fluorescence level of Na V 1.7-HEK cells with ANG-2 following the addition of Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1 mM) or Amaranth (1 mM) obtained using the FLIPR TETRA . (C) The average relative light units corresponding to 60–100 s after addition of quenchers or buffer. Reduction in background fluorescence by Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1mM) and Amaranth (1 mM) was not significantly different to washing. Statistical significance was determined using one-way ANOVA with Dunnett’s post-test, * P < 0.05 compared to wash. (D-H) Concentration-response curves for the reduction in background fluorescence of Na V 1.7-HEK cells with ANG-2 by quenchers Brilliant Black BN, Carmine, Ponceau 4R, Allura Red and Amaranth. Data are presented as mean ± SEM from three wells.
Hek293 Cells Heterologously Expressing Human Na V 1.7, supplied by SB Drug Discovery, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automated+patch-clamp+platform+qpatch+16/hek+hnav1+7+stable+cell+line/pmc06411159-55-7-15
Average 90 stars, based on 1 article reviews
hek293 cells heterologously expressing human na v 1.7 - by Bioz Stars, 2026-08
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human na v 1.7  (SB Drug Discovery)
90
SB Drug Discovery human na v 1.7
(A) Sample traces of the fluorescence levels of Na V <t>1.7-HEK</t> cells with ANG-2 and PSS buffer addition, with and without washing, obtained using the FLIPR TETRA . (B) Sample traces of the fluorescence level of Na V 1.7-HEK cells with ANG-2 following the addition of Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1 mM) or Amaranth (1 mM) obtained using the FLIPR TETRA . (C) The average relative light units corresponding to 60–100 s after addition of quenchers or buffer. Reduction in background fluorescence by Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1mM) and Amaranth (1 mM) was not significantly different to washing. Statistical significance was determined using one-way ANOVA with Dunnett’s post-test, * P < 0.05 compared to wash. (D-H) Concentration-response curves for the reduction in background fluorescence of Na V 1.7-HEK cells with ANG-2 by quenchers Brilliant Black BN, Carmine, Ponceau 4R, Allura Red and Amaranth. Data are presented as mean ± SEM from three wells.
Human Na V 1.7, supplied by SB Drug Discovery, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automated+patch-clamp+platform+qpatch+16/human+na+v+1+7/pmc08494138-125-24-28
Average 90 stars, based on 1 article reviews
human na v 1.7 - by Bioz Stars, 2026-08
90/100 stars
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qpatch 16 automated whole-cell patch clamp protocol  (CEREP Inc)
90
CEREP Inc qpatch 16 automated whole-cell patch clamp protocol
(A) Sample traces of the fluorescence levels of Na V <t>1.7-HEK</t> cells with ANG-2 and PSS buffer addition, with and without washing, obtained using the FLIPR TETRA . (B) Sample traces of the fluorescence level of Na V 1.7-HEK cells with ANG-2 following the addition of Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1 mM) or Amaranth (1 mM) obtained using the FLIPR TETRA . (C) The average relative light units corresponding to 60–100 s after addition of quenchers or buffer. Reduction in background fluorescence by Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1mM) and Amaranth (1 mM) was not significantly different to washing. Statistical significance was determined using one-way ANOVA with Dunnett’s post-test, * P < 0.05 compared to wash. (D-H) Concentration-response curves for the reduction in background fluorescence of Na V 1.7-HEK cells with ANG-2 by quenchers Brilliant Black BN, Carmine, Ponceau 4R, Allura Red and Amaranth. Data are presented as mean ± SEM from three wells.
Qpatch 16 Automated Whole Cell Patch Clamp Protocol, supplied by CEREP Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automated+patch-clamp+platform+qpatch+16/patch+clamp+assay/us09249147-1231-22-36
Average 90 stars, based on 1 article reviews
qpatch 16 automated whole-cell patch clamp protocol - by Bioz Stars, 2026-08
90/100 stars
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human nav1.8  (Chantest Inc)
90
Chantest Inc human nav1.8
(A) Sample traces of the fluorescence levels of Na V <t>1.7-HEK</t> cells with ANG-2 and PSS buffer addition, with and without washing, obtained using the FLIPR TETRA . (B) Sample traces of the fluorescence level of Na V 1.7-HEK cells with ANG-2 following the addition of Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1 mM) or Amaranth (1 mM) obtained using the FLIPR TETRA . (C) The average relative light units corresponding to 60–100 s after addition of quenchers or buffer. Reduction in background fluorescence by Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1mM) and Amaranth (1 mM) was not significantly different to washing. Statistical significance was determined using one-way ANOVA with Dunnett’s post-test, * P < 0.05 compared to wash. (D-H) Concentration-response curves for the reduction in background fluorescence of Na V 1.7-HEK cells with ANG-2 by quenchers Brilliant Black BN, Carmine, Ponceau 4R, Allura Red and Amaranth. Data are presented as mean ± SEM from three wells.
Human Nav1.8, supplied by Chantest Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automated+patch-clamp+platform+qpatch+16/cho+cells+stably+expressing+human+na+v+1+8/pm32524995-67-33-35
Average 90 stars, based on 1 article reviews
human nav1.8 - by Bioz Stars, 2026-08
90/100 stars
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flyscreen high-throughput patch-clamp system  (Flyion GmbH)
90
Flyion GmbH flyscreen high-throughput patch-clamp system
(A) Sample traces of the fluorescence levels of Na V <t>1.7-HEK</t> cells with ANG-2 and PSS buffer addition, with and without washing, obtained using the FLIPR TETRA . (B) Sample traces of the fluorescence level of Na V 1.7-HEK cells with ANG-2 following the addition of Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1 mM) or Amaranth (1 mM) obtained using the FLIPR TETRA . (C) The average relative light units corresponding to 60–100 s after addition of quenchers or buffer. Reduction in background fluorescence by Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1mM) and Amaranth (1 mM) was not significantly different to washing. Statistical significance was determined using one-way ANOVA with Dunnett’s post-test, * P < 0.05 compared to wash. (D-H) Concentration-response curves for the reduction in background fluorescence of Na V 1.7-HEK cells with ANG-2 by quenchers Brilliant Black BN, Carmine, Ponceau 4R, Allura Red and Amaranth. Data are presented as mean ± SEM from three wells.
Flyscreen High Throughput Patch Clamp System, supplied by Flyion GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automated+patch-clamp+platform+qpatch+16/flyscreen+high+throughput+patch+clamp+system/10__1038_slash_438703a-0-88-87
Average 90 stars, based on 1 article reviews
flyscreen high-throughput patch-clamp system - by Bioz Stars, 2026-08
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hna v 1.8/β3 in a tetracycline-inducible system  (Chantest Inc)
90
Chantest Inc hna v 1.8/β3 in a tetracycline-inducible system
(A) Sample traces of the fluorescence levels of Na V <t>1.7-HEK</t> cells with ANG-2 and PSS buffer addition, with and without washing, obtained using the FLIPR TETRA . (B) Sample traces of the fluorescence level of Na V 1.7-HEK cells with ANG-2 following the addition of Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1 mM) or Amaranth (1 mM) obtained using the FLIPR TETRA . (C) The average relative light units corresponding to 60–100 s after addition of quenchers or buffer. Reduction in background fluorescence by Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1mM) and Amaranth (1 mM) was not significantly different to washing. Statistical significance was determined using one-way ANOVA with Dunnett’s post-test, * P < 0.05 compared to wash. (D-H) Concentration-response curves for the reduction in background fluorescence of Na V 1.7-HEK cells with ANG-2 by quenchers Brilliant Black BN, Carmine, Ponceau 4R, Allura Red and Amaranth. Data are presented as mean ± SEM from three wells.
Hna V 1.8/β3 In A Tetracycline Inducible System, supplied by Chantest Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automated+patch-clamp+platform+qpatch+16/hna+v+1+8+%CE%B23+in+a+tetracycline+inducible+system/pmc07599555-112-70-72
Average 90 stars, based on 1 article reviews
hna v 1.8/β3 in a tetracycline-inducible system - by Bioz Stars, 2026-08
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Image Search Results


(A) Sample traces of the fluorescence levels of Na V 1.7-HEK cells with ANG-2 and PSS buffer addition, with and without washing, obtained using the FLIPR TETRA . (B) Sample traces of the fluorescence level of Na V 1.7-HEK cells with ANG-2 following the addition of Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1 mM) or Amaranth (1 mM) obtained using the FLIPR TETRA . (C) The average relative light units corresponding to 60–100 s after addition of quenchers or buffer. Reduction in background fluorescence by Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1mM) and Amaranth (1 mM) was not significantly different to washing. Statistical significance was determined using one-way ANOVA with Dunnett’s post-test, * P < 0.05 compared to wash. (D-H) Concentration-response curves for the reduction in background fluorescence of Na V 1.7-HEK cells with ANG-2 by quenchers Brilliant Black BN, Carmine, Ponceau 4R, Allura Red and Amaranth. Data are presented as mean ± SEM from three wells.

Journal: PLoS ONE

Article Title: Development of a high-throughput fluorescent no-wash sodium influx assay

doi: 10.1371/journal.pone.0213751

Figure Lengend Snippet: (A) Sample traces of the fluorescence levels of Na V 1.7-HEK cells with ANG-2 and PSS buffer addition, with and without washing, obtained using the FLIPR TETRA . (B) Sample traces of the fluorescence level of Na V 1.7-HEK cells with ANG-2 following the addition of Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1 mM) or Amaranth (1 mM) obtained using the FLIPR TETRA . (C) The average relative light units corresponding to 60–100 s after addition of quenchers or buffer. Reduction in background fluorescence by Brilliant Black BN (1 mM), Carmine (1 mM), Ponceau 4R (1 mM), Allura Red (1mM) and Amaranth (1 mM) was not significantly different to washing. Statistical significance was determined using one-way ANOVA with Dunnett’s post-test, * P < 0.05 compared to wash. (D-H) Concentration-response curves for the reduction in background fluorescence of Na V 1.7-HEK cells with ANG-2 by quenchers Brilliant Black BN, Carmine, Ponceau 4R, Allura Red and Amaranth. Data are presented as mean ± SEM from three wells.

Article Snippet: Automated whole-cell patch-clamp recordings were performed on HEK293 cells heterologously expressing human Na V 1.7 (SB Drug Discovery, Glasgow, UK) using a QPatch-16 automated electrophysiology platform (Sophion Bioscience, Ballerup, Denmark) as previously described [ ].

Techniques: Fluorescence, Concentration Assay